Appeal court judges on Tuesday upheld the ban imposed last year on No Surrender MC and Hells Angels MC.
Both biker clubs have a culture of violence which threatens public safety and their members are systematically involved in crime, such as drugs trading and blackmail, the appeal court said.
Last May, civil court judges in Utrecht agreed that the Dutch arm of Hells Angels MC should be banned and broken up, ending years of legal action by the public prosecution department.
In June 2019, judges in Assen banned the No Surrender MC, saying it presented a threat to public order.
The courts had earlier banned biker clubs Satudarah and Bandidos, although the Bandidos ban was partially overturned on appeal.